Modified comet assays for the detection of cyclobutane pyrimidine dimers and oxidative base damages

Modified comet assays for the detection of cyclobutane pyrimidine dimers and oxidative base damages

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The comet assay (also known as single-cell gel electrophoresis) is a technique for the detection of DNA damage at the level of the individual cell.It is a versatile, relatively simple to perform and sensitive mpu63zm/a method.Although most investigations make use of its ability to measure DNA single-strand breaks, modifications to the method allow detection of cyclobutane pyrimidine dimers (CPDs), crosslinks, base damage, and apoptotic nuclei.Many investigators also interested in examining the DNA swisse high strength magnesium powder berry damage as a function of time after exposure to a known genotoxic agent.Here, we present a procedure of comet assay for the detection of DNA strand breaks, base damages, and CPDs that can be used to measure DNA damage during toxicity, oxidative stress, and ultraviolet radiation exposure and it can be applied in human toxicological biomonitoring scenarios.